The Metabolism of Nucleotides
Before we look at nucleic acid structure, we need to study the synthesis of the purine and pyrimidine ribonucleotides and the subsequent synthesis of the deoxyribonucleotides, followed by their catabolic and recycling pathways. The synthetic pathways are particularly detailed, and I suggest that you review my introduction to the study of biochemical pathways so that you can break the information down into manageable pieces. Along the way, we will correlate the information, where appropriate, to disease states. In such instances, I will use the header "Clinical Correlate".
Purine Ribonucleotide Synthesis
Surprisingly, the purine ribonucleotides are formed as such de novo, and the bases themselves are derived from them. The first purine ribonucleotide to be synthesized in an organism is inosine monophosphate.
5'-Nucleotides are produced through the hydrolytic reaction from Ribonucleic acid(RNA) as raw material by enzymatic catalysis to form four kinds of nucleotides 5- 'AMP (adenosine-5'-monophosphate),5'-GMP•2Na(guanosine-5'-monophosphate,disodium salt),5'-CMP(cytidine-5'-monophosphate)-,5'-UMP•2Na(uridine-5'-monophosphate,Disodium salt) .By the chromatographic isolation, four kinds of separated 5'-nucleotide products with high purity can be obtained. These products can be widely applied in medicine, reagent, fine chemical industry, and additive of food fields etc.